Groovy Map containing sample information
e.g. [ id:‘test’, single_end:false ]
reads
:file
List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.
meta2
:map
Groovy Map containing reference information
e.g. [ id:‘genome’ ]
index
:file
HISAT2 genome index file
*.ht2
meta3
:map
Groovy Map containing reference information
e.g. [ id:‘genome’ ]
splicesites
:file
Splices sites in gtf file
*.{txt}
Output
name:type
description
pattern
bam
meta
:map
Groovy Map containing sample information
e.g. [ id:‘test’, single_end:false ]
*.bam
:file
Output BAM file containing read alignments
*.{bam}
summary
meta
:map
Groovy Map containing sample information
e.g. [ id:‘test’, single_end:false ]
*.log
:file
Alignment log
*.log
fastq
meta
:map
Groovy Map containing sample information
e.g. [ id:‘test’, single_end:false ]
*fastq.gz
:file
Output FastQ file
*fastq.gz
versions
versions.yml
:file
File containing software versions
versions.yml
Tools
hisat2
MIT
HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome.