Description

Align RNA-Seq reads to a reference with HISAT2

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2

:map

Groovy Map containing reference information e.g. [ id:‘genome’ ]

index

:file

HISAT2 genome index file

*.ht2

meta3

:map

Groovy Map containing reference information e.g. [ id:‘genome’ ]

splicesites

:file

Splices sites in gtf file

*.{txt}

Output

name:type
description
pattern

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bam

:file

Output BAM file containing read alignments

*.{bam}

summary

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.log

:file

Alignment log

*.log

fastq

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*fastq.gz

:file

Output FastQ file

*fastq.gz

versions

versions.yml

:file

File containing software versions

versions.yml

Tools

hisat2
MIT

HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome.